IDENTIFICATION OF AGAROLYTIC BACTERIA PDF
special ecological reason for the presence of agarolytic bacteria in fresh water but that Identification of Medical Bacteria, 2nd edn. Cam-. Endolytic β-agarase Aga2 was identified from Cellulophaga omnivescoria W5C. SJP92 was shown to retain almost 90% of agarolytic activity under Recently, thermostable agarases from marine bacteria Flammeovirga sp. Abstract: Agarolytic bacteria use agarase to utilize agar as sole source of carbon. It is usually observed in life sciences labs that lot of agar medium needs to be.
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The identidication of proteases into the medium during the stationary phase was demonstrated utilizing Azocoll Calbiochem-Behring, La Jolla, Calif. Previous results on the purification and characterization of an extracellular agarase from the agar-liquefying strain Alteromonas sp. We describe here the characterization of a new agarolytic bacterium isolated from the southern Chilean coast.
This strain was identified as P. On the basis of several phenotypic characters and a phylogenetic analysis of the genes coding for the 16S rRNA, this strain was identified as Pseudoalteromonas antarctica strain N Groleau D, Yaphe W.
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Abstract The phenotypic and agarolytic features of an unidentified marine bacteria that was isolated from the southern Pacific coast was investigated. It requires sodium ion for growth, has an oxidative metabolism, and does not accumulate polyhydroxybutyrate as an intracellular reserve. This effect would be related to the production of low-molecular-weight agarases that can diffuse though the gel pores. The enzyme was purified by taking advantage of its high binding affinity to DEAE-cellulose when loaded at low salt concentrations at cruder stages.
Isolation And Identification of agarolytic bacteria in Marin by Faizah Rahman on Prezi
Proc Int Seaweed Symp. In our laboratory, we have isolated a few agar-softening and agar-liquefying bacterial strains from the southern Chilean coast to characterize their extracellular agarases in an attempt to contribute to our understanding of the basis of agar hydrolysis.
Sugars were sterilized by filtration through 0. Cambridge University Press; Other biochemical and physiological tests were carried out essentially as described by Stolp and Gadkari 38 and Stanier et al. The C-1 signal in this case is observed at Cloning and sequencing of agaAa unique bactegia gene from a marine bacterium. The pH profile of agarase from strain N-1 identificatioon bell shaped, with a maximum at pH 7. The oligosaccharides were detected by evaluation of the refractive index.
Phylogenetic analysis of 16S rRNA. The sequence of the 16S rDNA of strain N-1 was aligned with the sequences of a number of Pseudoalteromonas strains available and was analyzed essentially as described by Gauthier et al. Barbeyron H, Kloareg B.
The agarase gene dagA of Streptomyces coelicolor A3 2: Strain N-1 can also be distinguished from S. The amounts of protein in the pooled fractions were estimated by the method of Bradford 12 with fructose-1,6-bisphosphatase as the standard.
K m values of 0. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. American Society for Microbiology; idehtification When enzyme activity agadolytic measured in the presence of NaCl in agaropytic of up to 0. In contrast to the agarases from P.
As shown by the HPLC profile, the purified enzyme from strain N-1 hydrolyzed agar to give two main oligosaccharide products Fig.
Purification and some properties of agarase from Pseudomonas sp. However, we must point out that strain N-1 differs from the type strain of this species in some properties. Manual of methods for general microbiology. Cell growth and activity measurements. Isolation and characterization of Cytophaga flevensis sp. Additional purification of the enzyme was achieved by gel filtration on Sephadex G75 Fig.
HPLC analysis of the hydrolysis products of unsubstituted agar generated by agarase from P. Journal List Appl Environ Microbiol v. A Effect of pH on the activity of the purified agarase.
Effect of carbon sources on bacterial growth and agarase production. Phenylmethylsulfonylfluoride PMSF was added to a final concentration of 0.